Responses save in this browser when storage is available. Download a copy before changing device. Nothing is submitted to MSH or a teacher.
Your learning targets
Locate xylem and phloem in root, stem and leaf sections and state their functions.
Trace water from soil to air and distinguish evaporation from diffusion.
Interpret transpiration data and explain source-to-sink transport in Extended work.
Suggested pacing: Session 1: starter 5 min, learn/watch 25 min, activities 15 min. Session 2: retrieval 5 min, quiz 10 min, exam practice 25 min, exit ticket 5 min. Pause or split further to suit your class.
Starter · retrieve and connect
Check the connection
Phloem transports sucrose and amino acids to parts that use or store them. Xylem carries water and mineral ions from roots and helps support the plant.
Learn the science
01 · Compare the transport tissues
Feature
Xylem
Phloem
Main substances
Water and mineral ions
Sucrose and amino acids
Role
Transport and support
Transport of organic nutrients
Typical transport direction
From roots towards leaves
From source to sink; can be up or down in different tubes
Useful structural link
Thick, lignified vessel walls help support the plant
Living tissue transports assimilates
Remember sucrose, not starch, is transported in phloem. Both tissues are found in vascular bundles. Detailed tissue structure is an application link, not an additional B8 requirement.
02 · Where are xylem and phloem?
Use position, not a memorised colour: real microscope images are not necessarily blue and orange. In a non-woody dicot root, xylem commonly forms a central star with phloem between its arms. In a stem, vascular bundles form a ring: xylem is towards the centre, phloem towards the outside. In a leaf vein, xylem is nearer the upper surface and phloem nearer the lower surface.
03 · Water enters at the root
A root hair cell is an epidermal cell with a long, narrow extension. This provides a large surface area in contact with soil water, increasing uptake of water and mineral ions. From B3: water enters by osmosis when soil water has a higher water potential than the cell sap; mineral-ion uptake can involve active transport.
Water enters a root hair cell.
It passes across root cortex cells.
It enters xylem and moves through root and stem towards the leaf.
It reaches mesophyll cells.
Osmosis refers to water crossing a partially permeable membrane. Do not describe the entire upward xylem flow as osmosis.
04 · Transpiration: from leaf to atmosphere
Transpiration is loss of water vapour from leaves. Liquid water evaporates from moist mesophyll-cell surfaces into the leaf’s air spaces. Water vapour then diffuses through open stomata into the outside air, down its concentration gradient.
As leaves lose water, water supplied by xylem replaces it. The resulting transpiration stream links the root supply to the leaf. The plant does not pump water using a heart.
Write the two stages separately: evaporation from mesophyll surfaces; diffusion of water vapour through stomata. Water does not “evaporate from the xylem straight out of the leaf”.
05 · What changes the rate?
Change, other factors constant
Usual effect
Explanation [Supplement]
Higher temperature
Rate increases over a suitable range
Faster evaporation and faster diffusion of water vapour.
Greater wind speed
Rate increases
Moving air removes humid air near the leaf, maintaining a steeper water-vapour concentration gradient.
Higher humidity [Supplement]
Rate decreases
Outside air contains more water vapour, so the gradient from leaf to air is smaller.
These comparisons assume water is available and stomata remain open. Under severe water stress, stomatal closure can change the pattern.
Wilting Supplement
If water loss exceeds uptake for long enough, cells lose water and turgor pressure falls. Soft tissues become less rigid; leaves and stems droop. Wilting is not the same as plasmolysis in every situation.
06 · Translocation Supplement
Translocation moves sucrose and amino acids in phloem from sources to sinks. A source releases these substances into transport; a sink uses them or stores them.
Situation
Source
Sink
Mature leaf supplying a growing root
Photosynthesising leaf
Growing root
Leaf supplying a developing fruit
Photosynthesising leaf
Fruit storing nutrients
Stored reserves mobilised during new growth
Storage organ
Growing shoot
A root is not always a sink: a storage root can become a source when its reserves are mobilised. “Source” and “sink” describe current function, not a permanent organ label.
Structure focus · recognise a root hair cell
Look for a single elongated cell with a narrow projection. It is not a whole root. The cell membrane lies just inside the wall; its partially permeable nature matters for osmosis.
Watch, pause and explain
Watch · Transpiration and translocation
Cognito · Open on YouTube. Internet access is required. If your school blocks the player, use the written lesson below.
Apply your understanding
Transpiration predictor · qualitative model
Predict before moving a control. This is an illustrative model, not measured plant data. Water is available and stomata are assumed open.
Index = 100 × (temperature ÷ 20) × [(wind + 1) ÷ 2] × [(100 − humidity) ÷ 50]. It is a teaching rule, not a physiological law. Baseline 20 °C, wind 1 and 50% humidity = 100.
Tissue and pathway sorter
Potometer calculator
A bubble moves along a capillary as a shoot takes up water. For a fixed capillary, bubble distance ÷ time is a useful uptake-rate proxy. It is not a direct measurement of all transpiration: some absorbed water is used or retained.
Quick quiz · 8 marks
One mark per correct answer. Select all eight answers, then check. You may change answers and check again for practice.
Not yet checked
Exam practice · 20 marks
Original Cambridge-style practice, not copied past-paper questions. Write first, then reveal mark points and self-assess. Written answers are not automatically graded; self-scores are not official grades.
Structured practice · original Cambridge-style questions
Reveal answer / mark points
Suggested marking: one mark per valid point, up to 3.
Xylem transports water and mineral ions.
Xylem provides support.
Phloem transports sucrose and amino acids.
Reveal answer / mark points
Suggested marking: one mark per valid point, up to 2.
Their long extensions provide a large surface area.
This increases water uptake / water enters by osmosis down a water-potential gradient.
Reveal answer / mark points
Suggested marking: one mark per valid point, up to 3.
Water evaporates from mesophyll-cell surfaces.
Water vapour enters the leaf air spaces.
It diffuses out through stomata.
Reveal answer / mark points
Suggested marking: one mark per valid point, up to 2.
Moving air removes humid air next to the leaf.
This maintains a steeper water-vapour concentration gradient, increasing diffusion.
Practical skills · evaluate a potometer investigation
Illustrative results using the same shoot and capillary; temperature, humidity and light are kept constant.
Fan setting
Trial 1 / mm min⁻¹
Trial 2 / mm min⁻¹
Trial 3 / mm min⁻¹
Off
2.0
2.2
1.8
Low
3.9
4.1
4.0
High
6.1
5.9
6.0
Reveal answer / mark points
Suggested marking: one mark per valid point, up to 3.
Suggested marking: one mark per valid point, up to 2.
Allow the shoot to acclimatise at each setting / measure wind speed with an anemometer.
Check the apparatus is airtight / repeat with more shoots while controlling leaf area.
Reveal answer / mark points
Suggested marking: one mark per valid point, up to 2.
Some absorbed water is retained in cells / used in photosynthesis or growth.
Therefore a potometer estimates uptake, not water-vapour loss directly.
Reveal answer / mark points
Suggested marking: one mark per valid point, up to 3.
Water loss exceeds uptake.
Cells lose water, reducing turgor pressure.
Soft tissues lose support and droop.
Teacher-led setup: cut the shoot under water to reduce air entry; seal joints; handle glass and blades safely. This page uses supplied data and requires no practical equipment.
Finish and reflect
Exit ticket
Sources and teacher notes
This lesson follows Cambridge 0654 syllabus for exams in 2025–2027, B8.1–B8.4. The verified sequence after B6 is B7 Human nutrition, B8 Transport in plants, B9 Transport in animals. Check the examination-year syllabus for other cohorts.
Official topic order and video pages checked on 14 September 2026 (Bermuda). Embedded videos are hosted by YouTube and require internet access; playback depends on regional and school settings. All written learning, diagrams and activities work without a network connection.
Independent MSH teaching resource; not endorsed by Cambridge International. Scientific diagrams are original schematics, not to scale. Supplement labels identify Extended work; application boxes may include supporting explanation beyond the specified minimum.
Responses remain on the current browser/device when local storage is permitted. Use the download button for a readable text record. No backend, login, tracking or gradebook submission is included.