MYERS SCIENCE HUBCambridge IGCSE Co-ordinated Sciences · 0654
Biology · B8 · Interactive lesson

Transport in plants

Two transport tissues. Two different journeys.

Core + labelled SupplementB8.1–B8.4Two 45-minute sessions + optional consolidation

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Your learning targets

  • Locate xylem and phloem in root, stem and leaf sections and state their functions.
  • Trace water from soil to air and distinguish evaporation from diffusion.
  • Interpret transpiration data and explain source-to-sink transport in Extended work.

Suggested pacing: Session 1: starter 5 min, learn/watch 25 min, activities 15 min. Session 2: retrieval 5 min, quiz 10 min, exam practice 25 min, exit ticket 5 min. Pause or split further to suit your class.

Starter · retrieve and connect

Check the connection

Phloem transports sucrose and amino acids to parts that use or store them. Xylem carries water and mineral ions from roots and helps support the plant.

Learn the science

01 · Compare the transport tissues

FeatureXylemPhloem
Main substancesWater and mineral ionsSucrose and amino acids
RoleTransport and supportTransport of organic nutrients
Typical transport directionFrom roots towards leavesFrom source to sink; can be up or down in different tubes
Useful structural linkThick, lignified vessel walls help support the plantLiving tissue transports assimilates
Remember sucrose, not starch, is transported in phloem. Both tissues are found in vascular bundles. Detailed tissue structure is an application link, not an additional B8 requirement.

02 · Where are xylem and phloem?

RootStemLeaf veinUpper surfaceXylemPhloemSchematic sections · non-woody dicotyledonous plantRoot: centre · Stem: xylem inward · Leaf: xylem above

Use position, not a memorised colour: real microscope images are not necessarily blue and orange. In a non-woody dicot root, xylem commonly forms a central star with phloem between its arms. In a stem, vascular bundles form a ring: xylem is towards the centre, phloem towards the outside. In a leaf vein, xylem is nearer the upper surface and phloem nearer the lower surface.

03 · Water enters at the root

A root hair cell is an epidermal cell with a long, narrow extension. This provides a large surface area in contact with soil water, increasing uptake of water and mineral ions. From B3: water enters by osmosis when soil water has a higher water potential than the cell sap; mineral-ion uptake can involve active transport.

  1. Water enters a root hair cell.
  2. It passes across root cortex cells.
  3. It enters xylem and moves through root and stem towards the leaf.
  4. It reaches mesophyll cells.
Osmosis refers to water crossing a partially permeable membrane. Do not describe the entire upward xylem flow as osmosis.

04 · Transpiration: from leaf to atmosphere

Transpiration is loss of water vapour from leaves. Liquid water evaporates from moist mesophyll-cell surfaces into the leaf’s air spaces. Water vapour then diffuses through open stomata into the outside air, down its concentration gradient.

As leaves lose water, water supplied by xylem replaces it. The resulting transpiration stream links the root supply to the leaf. The plant does not pump water using a heart.

Write the two stages separately: evaporation from mesophyll surfaces; diffusion of water vapour through stomata. Water does not “evaporate from the xylem straight out of the leaf”.

05 · What changes the rate?

Change, other factors constantUsual effectExplanation [Supplement]
Higher temperatureRate increases over a suitable rangeFaster evaporation and faster diffusion of water vapour.
Greater wind speedRate increasesMoving air removes humid air near the leaf, maintaining a steeper water-vapour concentration gradient.
Higher humidity [Supplement]Rate decreasesOutside air contains more water vapour, so the gradient from leaf to air is smaller.

These comparisons assume water is available and stomata remain open. Under severe water stress, stomatal closure can change the pattern.

Wilting Supplement

If water loss exceeds uptake for long enough, cells lose water and turgor pressure falls. Soft tissues become less rigid; leaves and stems droop. Wilting is not the same as plasmolysis in every situation.

06 · Translocation Supplement

Translocation moves sucrose and amino acids in phloem from sources to sinks. A source releases these substances into transport; a sink uses them or stores them.

SituationSourceSink
Mature leaf supplying a growing rootPhotosynthesising leafGrowing root
Leaf supplying a developing fruitPhotosynthesising leafFruit storing nutrients
Stored reserves mobilised during new growthStorage organGrowing shoot

A root is not always a sink: a storage root can become a source when its reserves are mobilised. “Source” and “sink” describe current function, not a permanent organ label.

Structure focus · recognise a root hair cell

VacuoleLong root hair extensionLarge surface areaCell wallNucleusCytoplasm around vacuole

Look for a single elongated cell with a narrow projection. It is not a whole root. The cell membrane lies just inside the wall; its partially permeable nature matters for osmosis.

Watch, pause and explain

Watch · Transpiration and translocation

Cognito · Open on YouTube. Internet access is required. If your school blocks the player, use the written lesson below.

Apply your understanding

Transpiration predictor · qualitative model

Predict before moving a control. This is an illustrative model, not measured plant data. Water is available and stomata are assumed open.

Index = 100 × (temperature ÷ 20) × [(wind + 1) ÷ 2] × [(100 − humidity) ÷ 50]. It is a teaching rule, not a physiological law. Baseline 20 °C, wind 1 and 50% humidity = 100.

Tissue and pathway sorter

Potometer calculator

A bubble moves along a capillary as a shoot takes up water. For a fixed capillary, bubble distance ÷ time is a useful uptake-rate proxy. It is not a direct measurement of all transpiration: some absorbed water is used or retained.

Quick quiz · 8 marks

One mark per correct answer. Select all eight answers, then check. You may change answers and check again for practice.

1. Which substance is normally transported in phloem?
2. What is the correct water pathway?
3. Where does water evaporate inside a leaf?
4. What process moves water vapour out through stomata?
5. Increasing humidity usually…
6. In a dicot stem bundle, xylem is usually…
7. A growing fruit using sucrose is a…
8. A potometer most directly estimates…

Not yet checked

Exam practice · 20 marks

Original Cambridge-style practice, not copied past-paper questions. Write first, then reveal mark points and self-assess. Written answers are not automatically graded; self-scores are not official grades.

Structured practice · original Cambridge-style questions

Reveal answer / mark points

Suggested marking: one mark per valid point, up to 3.

  • Xylem transports water and mineral ions.
  • Xylem provides support.
  • Phloem transports sucrose and amino acids.
Reveal answer / mark points

Suggested marking: one mark per valid point, up to 2.

  • Their long extensions provide a large surface area.
  • This increases water uptake / water enters by osmosis down a water-potential gradient.
Reveal answer / mark points

Suggested marking: one mark per valid point, up to 3.

  • Water evaporates from mesophyll-cell surfaces.
  • Water vapour enters the leaf air spaces.
  • It diffuses out through stomata.
Reveal answer / mark points

Suggested marking: one mark per valid point, up to 2.

  • Moving air removes humid air next to the leaf.
  • This maintains a steeper water-vapour concentration gradient, increasing diffusion.

Practical skills · evaluate a potometer investigation

Illustrative results using the same shoot and capillary; temperature, humidity and light are kept constant.

Fan settingTrial 1 / mm min⁻¹Trial 2 / mm min⁻¹Trial 3 / mm min⁻¹
Off2.02.21.8
Low3.94.14.0
High6.15.96.0
Reveal answer / mark points

Suggested marking: one mark per valid point, up to 3.

  • High mean = (6.1 + 5.9 + 6.0) ÷ 3 = 6.0 mm min⁻¹.
  • Fan-off mean = 2.0 mm min⁻¹.
  • Percentage increase = (6.0 − 2.0) ÷ 2.0 × 100 = 200%.
Reveal answer / mark points

Suggested marking: one mark per valid point, up to 2.

  • Allow the shoot to acclimatise at each setting / measure wind speed with an anemometer.
  • Check the apparatus is airtight / repeat with more shoots while controlling leaf area.
Reveal answer / mark points

Suggested marking: one mark per valid point, up to 2.

  • Some absorbed water is retained in cells / used in photosynthesis or growth.
  • Therefore a potometer estimates uptake, not water-vapour loss directly.
Reveal answer / mark points

Suggested marking: one mark per valid point, up to 3.

  • Water loss exceeds uptake.
  • Cells lose water, reducing turgor pressure.
  • Soft tissues lose support and droop.

Teacher-led setup: cut the shoot under water to reduce air entry; seal joints; handle glass and blades safely. This page uses supplied data and requires no practical equipment.

Finish and reflect

Exit ticket

Sources and teacher notes

This lesson follows Cambridge 0654 syllabus for exams in 2025–2027, B8.1–B8.4. The verified sequence after B6 is B7 Human nutrition, B8 Transport in plants, B9 Transport in animals. Check the examination-year syllabus for other cohorts.

Official topic order and video pages checked on 14 September 2026 (Bermuda). Embedded videos are hosted by YouTube and require internet access; playback depends on regional and school settings. All written learning, diagrams and activities work without a network connection.

Independent MSH teaching resource; not endorsed by Cambridge International. Scientific diagrams are original schematics, not to scale. Supplement labels identify Extended work; application boxes may include supporting explanation beyond the specified minimum.

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Continue the series: B7 Human nutrition · B8 Transport in plants · B9 Transport in animals