AP Biology Unit 1.1: Properties of Water

Hydrogen Bonding, Polarity & Emergent Properties of Water

Investigation focus

AP Biology focus: Explain how the polarity of water molecules and hydrogen bonding contribute to properties of water that are essential for biological systems.

This lab uses observable phenomena to connect molecular structure to macroscopic properties. You are expected to explain what happens using polarity, partial charges, and hydrogen bonding—not simply describe what you see.

polarity hydrogen bond cohesion adhesion surface tension capillary action specific heat evaporative cooling

Safe materials only

This version intentionally excludes biological fluids, stomach contents, battery acid, and other hazardous or inappropriate materials.

Suitable materials: water, salt water, sugar water, dish-soap solution, vegetable oil, food colouring, paper towel, coins/pennies, capillary tubes or narrow straws, ice, and room-temperature containers.

Student information

Pre-lab molecular model

1. Why is a water molecule polar?

2. What is a hydrogen bond in water?

3. Predict how disrupting hydrogen bonding might change the behavior of water.

Station 1 — Cohesion & Surface Tension

Coin-drop investigation
Molecular idea: Hydrogen bonds cause water molecules to attract one another. This cohesion contributes to surface tension.
  1. Place a clean, dry coin on a flat surface.
  2. Add water one drop at a time to the center of the coin. Count the drops until the dome collapses.
  3. Repeat for three trials.
  4. Repeat the same procedure using dilute dish-soap solution.
LiquidTrial 1Trial 2Trial 3Average dropsObservation
Water —
Dilute soap solution —

AP-level analysis: Why did adding soap change the number of drops the coin could hold?

Station 2 — Adhesion & Capillary Action

Paper towel / narrow tube investigation
Molecular idea: Water is attracted both to itself (cohesion) and to polar surfaces (adhesion). Together, these interactions contribute to capillary action.
  1. Place colored water in a beaker or cup.
  2. Insert one end of a paper towel strip or narrow capillary tube into the water.
  3. Observe the movement of water upward over time.
  4. Record the height reached at the specified times.
TimeHeight of water (cm)Observation
1 min
3 min
5 min

AP-level analysis: Explain how both cohesion and adhesion contributed to the upward movement of water.

Station 3 — Water as a Polar Solvent

Solubility comparison
Molecular idea: Because water is polar, it interacts strongly with ions and many polar molecules. Nonpolar substances do not interact with water in the same way.
  1. Add a small, equal amount of each test substance to separate cups of water.
  2. Stir each for the same amount of time.
  3. Record whether the substance dissolves, partially dissolves, or remains separate.
Substance added to waterPredictionResultMolecular explanation
Table salt
Sugar
Vegetable oil

AP-level analysis: Why is the phrase “like dissolves like” useful, but incomplete, when explaining water as a biological solvent?

Station 4 — Ice, Density & Hydrogen Bonding

Solid vs liquid water
Molecular idea: In ice, hydrogen bonds stabilize an open crystalline arrangement that spaces water molecules farther apart than in liquid water.
  1. Place one ice cube into a clear container of liquid water.
  2. Observe whether the ice sinks or floats.
  3. Record what the observation indicates about relative density.
ObservationDensity inferenceHydrogen-bond explanation

Biological significance: Why is it important that ice is less dense than liquid water?

Optional extension — Temperature Stability & Evaporative Cooling

Use this section if your teacher includes a thermal-properties station.

Water has a relatively high specific heat. How does hydrogen bonding help explain this property?

How does evaporation cool a surface?

Synthesis — From Molecular Structure to Biological Function

1. Which observed property depended most clearly on cohesion? Use evidence from your data.

2. How can the same molecular feature—water polarity—help explain both capillary action and solubility?

3. Choose one property investigated and explain why it matters for living organisms.

4. Construct a cause-and-effect chain from oxygen's electronegativity to one emergent property of water.

AP Biology CER Conclusion

Write a Claim–Evidence–Reasoning conclusion explaining how hydrogen bonding gives rise to the observed properties of water.

Completion status

Complete the observations and AP-level explanations. Your work can be saved locally on this device.

Submit to Myers Science Hub

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