Microbiology Practical Lab Form

Student information

Full name *

School email *

Class *

Lab date *

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Safety: Work only under teacher supervision using approved low-risk teaching strains and your school's microbiology procedures. Keep plates closed after incubation unless instructed otherwise, disinfect the work area, and dispose of cultures as directed by your teacher.

I have read and will follow the laboratory safety instructions. *

1

Preparing Agar Plates

Objective: Create sterile nutrient agar plates for bacterial cultivation.

Procedure reference

Weigh 2.8 g agar powder and add to 100 mL distilled water.
Sterilize by autoclaving at 121°C for 15 minutes.
Carefully pour into sterile Petri dishes using teacher-approved aseptic technique.
Allow plates to solidify for about 30 minutes.
Store plates inverted as directed by your teacher.

Data & observations

Agar mass used (g) *

Distilled water (mL) *

Number of plates prepared *

Autoclave temperature (°C) *

Autoclave time (min) *

Solidification time (min) *

Appearance of prepared agar plates * Evidence of contamination after storage/inspection * Activity 1 notes / deviations from the method

2

Streak Plate Method

Objective: Isolate individual bacterial colonies using aseptic technique.

Procedure reference

Sterilize the inoculating loop as directed.
Collect bacteria from the approved broth culture.
Streak Zone 1.
Re-sterilize/cool the loop and streak Zone 2 from Zone 1.
Repeat for Zone 3.
Incubate at 25°C for 24–48 hours, or as directed by your teacher.

Culture information

Organism / culture label *

Incubation temperature (°C) *

Incubation time (h) *

Growth by streak zone

Zone	Amount of growth *	Approx. colony count	Isolation quality *	Observation
Zone 1
Zone 2
Zone 3

Colony morphology

Colour *

Shape *

Margin *

Elevation

Surface / texture

Approx. colony diameter (mm)

Overall interpretation of streak plate *

3

Gram Staining

Objective: Differentiate bacteria based on cell wall structure.

Procedure reference

Prepare and heat-fix a bacterial smear.
Apply crystal violet for 60 s, then rinse.
Apply Gram's iodine for 60 s, then rinse.
Decolorize briefly with alcohol, then rinse immediately.
Apply safranin for 60 s, rinse and blot dry.
Observe using oil immersion at approximately 1000× total magnification.

Specimen / colony label *

Total magnification *

Final observed colour *

Cell shape *

Cell arrangement *

Gram reaction *

Microscopic observations * Interpretation *

Discussion Questions — Activities 1–3

Choose the best answer for each question. These are the discussion questions from the original Myers Science Hub virtual microbiology laboratory for Activities 1–3.

Discussion responses saved: 0 / 15

Final review & submission

Overall conclusion *

I confirm that the observations and responses recorded here are my own work. *

Ready to submit to the MSH Quiz backend.